Composite

Part:BBa_K2557027:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


Ubc promoter--TP901-RDF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1998
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1998
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 756
    Illegal BglII site found at 1057
    Illegal BglII site found at 1651
    Illegal BglII site found at 1777
    Illegal BglII site found at 2004
    Illegal BamHI site found at 408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1998
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1998
    Illegal AgeI site found at 414
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1254
    Illegal SapI.rc site found at 2198


Design Notes

In order to allow the recombinase to enter the nucleus and function, we added SV40 NLS to the front end. To detect protein content, we added the FLAG tag.


Source

The Ubc promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the sequence of TP901-RDF from the literature and synthesized this sequence.

References